Mohsen Akhavan Sepahi; Hossein Saghafi; Seyyed Majid Mosavi Movahhed; Mohammadreza Hayeri; Mohammad Ghareh Bagloo; Seyyed Mohammadreza Shokrollahi; Javad Hakimollahi; Zahra Movahhedi; Masoud Hooshmand
Volume 22, Issue 5 , November and December 2015, , Pages 740-747
Abstract
Background and Objective: Polycystic kidney disease (PKD) is a common inherited disease which makes renal cortex and medulla full of cysts and eventually lead to ESRD during middle age and then. This disorder is inherited as an autosomal dominant or recessive. ADPKD is common among adults .In this type ...
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Background and Objective: Polycystic kidney disease (PKD) is a common inherited disease which makes renal cortex and medulla full of cysts and eventually lead to ESRD during middle age and then. This disorder is inherited as an autosomal dominant or recessive. ADPKD is common among adults .In this type of inheritance, mutation occurs in one of three known genes. In almost 85% of patients, mutation occurred in PKD1 gene and 10-15% patients in PKD2 gene. Also the third gene has been identified as PKD3 which causes autosomal dominant family polycystic kidney, but the frequency of families carrying this mutation in this gene is reported very low.
Materials and Methods: Obtaining DNA performed in seven families with polycystic disease of adults and five members of these families. The obtained DNA was amplified using PCR and the continuous microsatellite to pathogenic type 1 and 2 gene were investigated. Microsatellite polymorphism which existed in these patients were selected and the assessment of gene linkage for all members of the household were used.
Results: Among the seven studied families, only the one with autosomal recessive inheritance in their pedigree, for D6S1344 marker was reported informative. In four other families with autosomal dominant inheritance, one of the D16S283, KG8 and D4S423 markers were positive. The remaining two families were not positive for any of the markers.
Conclusion: According to the findings of this study, five of seven studied families with genetic linkage analysis in terms of used markers were positive. It is suggested that for all kidney donors who have family history of ADPKD, genetic evaluation is done to assess their health. Besides in patients with a positive family history ADPKD, using analyzed continuous microsatellite in terms of mutations in PKD1 and PKD2 genes can achieve early diagnosis.
Sam Hatami; Javad Tafarroji; Mohmmad Ghareh Bagloo; Alireza Jalali; Aliakbar Riyahin; Mostafa Vahedian
Volume 21, Issue 3 , July and August 2014, , Pages 386-392
Abstract
Background: Understanding the epidemiology and main leading factors of endocarditis, accelerate the process of diagnosis and treatment, and reduce mortality from this disease. This study was performed due to uncertain epidemiological situation of endocarditis in different parts of Iran, especially city ...
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Background: Understanding the epidemiology and main leading factors of endocarditis, accelerate the process of diagnosis and treatment, and reduce mortality from this disease. This study was performed due to uncertain epidemiological situation of endocarditis in different parts of Iran, especially city of Qom.
Materials and Methods: In this cross-sectional study, epidemiological status of hospitalized patients with endocarditis in Qom city hospitals between 2004-2013 was reviewed. In this study, 74 patients who had duke criteria for infective endocarditis, were studied. Demographic data and the disease information were recorded on a Check list. Data collection method was the patients' hospital records. Data were analyzed using SPSS software version 16.
Results: In this study, 74 patients, including 49 males and 25 females were studied. The patients' mean age ± SD was 28 ± 15/78. According to information obtained, age range 20-23 years with 29 patients (39/1%), was the most affected. The most common cause of hospital admission was fever with 80% (59 patients). Tricuspid valve involvement was 67.5% (29 patients). The most common underlying disease was heart diseases by as much as 44/5% (33 patients). 42/4 % (14 patients) had a history of mitral valve replacement. Among those who had positive cultures, 85/7% (12 patients) Staphylococcus aureus, and 14/2% (2 paients) Klebsiella were reported. Mortality rate was 7.5%.
Conclusion: Fever, if accompanied with a heart murmur, or a history of mitral valve replacement in young men who have had a history of intravenous drug abuse, strongly suspected endocarditis.